Primer Premier's search algorithm finds optimal PCR, multiplex and SNP genotyping primers with the most accurate melting temperature using the nearest neighbor thermodynamic algorithm. Primers are screened for secondary structures, dimers, hairpins, homologies and physical properties before reporting the best ones for your sequence, in ranked order. Equipped with a handy calculator, you can easily manipulate sequences and analyze the results of your primer design.
Load the gene of interest from NCBI, select a search range, sit back and let Primer Premier pick the best possible primers for you.
What is new in this release:
Latest changes to the genomic databases of NCBI-BLAST along with fixes to reported problems are accommodated in this version.
What is new in version 6.20:
The upgrade accommodates the latest genomic databases available at the NCBI BLAST server and fixes to reported problems.